Fig. 6: Ahed deficiency induces cellular apoptosis.
From: A newly identified gene Ahed plays essential roles in murine haematopoiesis
a Number of cells yielded on day 5 culture of the FL-derived LSK cells. b Flow cytometry contour plots showing live (PI−, Annexin V−), early apoptotic (PI−, Annexin V+), late apoptotic (PI+, Annexin V+), and necrotic (PI+, Annexin V−) cell fractions on day 5 culture of the FL-derived LSK cells (left). The CD11b+ fraction was analysed. Percentages of cells in each quadrant are shown (right). c DNA content of cultured cells on day 5 culture of the FL-derived LSK cells (left). The proportions of apoptotic cells (DNA content < 2n) and cells in S-G2/M phase are shown (right). Data are presented as mean ± s.d. Statistical significance in a, c was determined by two-sided unpaired Student’s t-test, and in b by one-way ANOVA with Brown-Forsythe test. ***p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.
