Fig. 7: Pan-haematopoietic Ahed deletion disrupts critical gene expression.

a Differentially expressed genes from RNA-seq in foetal liver (FL) were compared with those in adult bone marrow (BM), in which gene expressions of Vav1-cre Ahed^fl/fl (cKO) versus Vav1-cre Ahed^fl/+ (control) in E14.5 FL, and R26CE Ahed^fl/fl (cKO) versus R26CE Ahed^fl/+ (control) mice in BM were analysed. A strong positive correlation was observed between the two groups. The Venn diagram shows the number of differentially expressed genes that overlapped between the two groups. b The top 10 enriched biological functional categories in which gene expressions of cKO versus control conducted in E14.5 FL (left) and adult BM (right), according to the Ingenuity Knowledge Base. The x-axis shows the significance, which is the value of –log (p value). c Upstream transcriptional regulators in Ahed cKO FLs compared with control FL (left), and in R26CE Ahed^fl/fl (cKO) BM compared with control BM (right). d Expression levels of representative haematopoietic stem cell-related genes, Gata2, Lmo2, Runx1 and Tal1 in E14.5 foetal liver LSK cells. Data are shown as mean expression levels ± s.d. a.u., arbitrary unit. Vav1-cre Ahed^fl/+ (n = 5); Vav1-cre Ahed^fl/fl (n = 6). e List of haematopoietic neoplasm patients with C11orf57 mutations from the database (COSMIC; https://cancer.sanger.ac.uk/cosmic/gene/analysis); somatic mutations of Ahed were found in some patients with haematopoietic neoplasm such as acute myeloid leukaemia, myelodysplastic syndrome, and chronic lymphocytic leukemia. f List of patients with C11orf57 mutations from the database, including splicing variant patterns (St. Jude Cloud PeCan; https://pecan.stjude.cloud/proteinpaint/). Statistical significance in b, c was determined by the Fisher’s Exact Test (conducted with Ingenuity Pathway Analysis software (Ingenuity Systems; Qiagen)), and in d by two-sided unpaired Student’s t-test. *p < 0.05. NS, not significant. Source data are provided as a Source Data file.