Fig. 1: Generation of fluorescently labeled reporter lines for visualizing microtubules in pollen.

a–d Representative images of 15 images showing the subcellular localizations of C-terminal GFP-tagged TUA1, TUB1, TUB4, and TUB9 in pollen grains and tubes. The fusion proteins were expressed under the control of native promoters in the corresponding mutant background, as indicated. The nuclei of VN and SCs in pollen grains and tubes were stained with DAPI. Bar in the upper panel, 10 μm; bar in the lower panel, 5 μm. e–h Representative images of 15 images showing the subcellular localizations of N-terminal mScarlet-tagged TUA1, TUB1, TUB4, and TUB9 in pollen grains and tubes. The fusion proteins were expressed under the control of native promoters in the corresponding mutant background, as indicated. The nuclei of VN and SCs in pollen grains and tubes were stained with DAPI. Bar in the upper panel, 10 μm; bar in the lower panel, 5 μm. i–k Representative images of 15 images showing the subcellular localizations of N-terminal mCherry-tagged AtEB1a, AtEB1b, and AtEB1c under the control of Lat52 in pollen grains and tubes. The nuclei of VN and SCs in pollen grains and tubes were stained with DAPI. Bar in the upper panel, 10 μm; bar in the lower panel, 5 μm. l Representative images of 20 images showing the subcellular localizations of the N-terminal mCherry-tagged microtubule-binding ___domain of Arabidopsis MAP4 (MBD) under the control of Lat52 in pollen grains and tubes. Bar in the upper panel, 10 μm; bar in the lower panel, 5 μm. m Representative images of 20 images showing the subcellular localization of microtubules in pollen grains and tubes revealed by indirect immunofluorescence assay using the antibody against β-tubulin. Bar in the upper panel, 10 μm; bar in the lower panel, 5 μm.