Fig. 4: Yng1EBM enhances DNA binding activity of Taf14.
From: Molecular insight into interactions between the Taf14, Yng1 and Sas3 subunits of the NuA3 complex
a, b EMSAs of 601 DNA in the presence of increasing amounts of Taf14FL (a) or Taf14ET (b) with or without Yng1EBM. DNA:protein ratio is shown below gel images. Experiments in (a, b) were performed once and twice, respectively, see also Supplementary Fig. 6. c Rotated views of the Taf14ET-Yng1EBM complex shown in Fig. 3a and colored according to its electrostatic potential (positive potential, blue; negative potential, red). An extended positively charged patch on the surface is outlined by green oval. d Binding curves used to determine apparent Kd for the interaction of Taf14FL with Yng1EBM in the presence of H3K9cr peptide at a 1:10 Taf14FL:H3K9cr molar ratio by tryptophan fluorescence (using a 1:1 stoichiometry). The apparent Kd value represents average of three independent measurements, with error calculated as SD between the runs. e A model for the formation of the complex between two molecules of Taf14 and two proteins containing EBM. H3K9acyl and DNA, the known ligands of the YEATS ___domain of Taf14, and the linker of Taf14, respectively, are indicated. f Overlap in genes with promoters bound by Taf14 and Yng1. g GO and KEGG category enrichments of genes with promoters co-occupied by Taf14 and Yng1. Circle size represents the approximate number of genes in the enrichment pathway. The exact number of genes is shown for the top pathway in each GO/KEGG category. h Genomic distribution of Taf14, Yng1, and Sas3 binding sites. Source data are provided as a Source Data file.
