Fig. 2: HPV18 E7 upregulates WWTR1 transcription.
From: The Hippo pathway transcription factors YAP and TAZ play HPV-type dependent roles in cervical cancer
A Representative western blots of HeLa cells after transfection with HPV18 E6/E7 targeting siRNA. Cell lysates were probed for TAZ, YAP, HPV18 E6 and HPV18 E7 expression. GAPDH was used as a loading control (n = 4). B RT-qPCR analysis of WWTR1 expression in HeLa cells after transfection with HPV18 E6/E7 targeting siRNA (n = 3). U6 was used as a loading control. C WWTR1-promoter luciferase assay in HeLa cells after transfection with HPV18 E6/E7 targeting siRNA (n = 3). D Representative western blot of C33A cell lysates stably expressing either HA-HPV18 E6 or HA-HPV18 E7 probed for TAZ, YAP, HPV18 E6, HPV18 E7 and the loading control GAPDH (n = 3). E RT-qPCR analysis of WWTR1 expression in C33A cells stably expressing either HA-HPV18 E6 or HA-HPV18 E7 (n = 3). U6 was used as a loading control. F WWTR1-promoter luciferase assay in C33A cells stably expressing either HA-HPV18 E6 or HA-HPV18 E7 (n = 3). G Representative western blot of C33A cell lysate stably expressing either HA-HPV18 E6 or HA-HPV18 E7 probed for TAZ, YAP, HPV16 E7, HPV18 E7 and GAPDH (n = 3). H RT-qPCR analysis of WWTR1 expression in C33A cells stably expressing either HA-HPV16 E7 or HA-HPV18 E7 (n = 4). U6 was used as a loading control. I WWTR1-promoter luciferase assay in C33A cells stably expressing either HA-HPV16 E7 or HA-HPV18 E7 (n = 3). Error bars represent the mean +/- standard deviation of a minimum of three biological repeats. *P < 0.05, **P < 0.01, ***P < 0.005 (two-tailed, unpaired Student’s t-test). Source data are provided as a Source Data file.
