Fig. 4: Inhibition of TAZ reduces HPV18+ cervical cancer cell proliferation.
From: The Hippo pathway transcription factors YAP and TAZ play HPV-type dependent roles in cervical cancer
A Immunofluorescence microscopy analysis of TAZ localisation (green) in HeLa cells treated with 6079510 or Ivermectin. DAPI stained nuclei (blue). Arrows point to nuclear TAZ. Scale bar 10 µm (n = 3). B Immunofluorescence microscopy analysis of nuclear YAP (green) in HeLa cells treated with 6079510 or Ivermectin. DAPI stained nuclei (blue). Arrows point to nuclear YAP. Scale bar 10 µm (n = 3). C Growth curve analysis of HeLa cells treated with 6079510 or Ivermectin (n = 3). D Colony formation assay of HeLa cells treated with 6079510 or Ivermectin (n = 4). E Soft agar assay of HeLa cells treated with 6079510 or Ivermectin (n = 3). F Growth curve analysis of SiHa cells treated with 6079510 or Ivermectin (n = 3). G Colony formation assay of SiHa cells treated with 6079510 or Ivermectin (n = 4). H Soft agar assay of SiHa cells treated with 6079510 or Ivermectin (n = 3). I RT-qPCR analysis of WWTR1 or YAP1 expression in TAZ KD HeLa cells (n = 3). U6 was used as a loading control. J Representative western blot of TAZ KD HeLa cells probed for TAZ, HPV18 E6, HPV18 E7 and GAPDH (n = 3). K Growth curve analysis of TAZ KD HeLa cells (n = 3). L Colony formation assay in TAZ KD HeLa cells (n = 4). M Soft agar assay in TAZ KD HeLa cells (n = 4). N Colony formation assay in C33A cells stably expressing HA-HPV18 E7 plus 6079510 treatment (n = 4). O Colony formation assay in HaCaT cells stably expressing HA-HPV18 E7 plus 6079510 treatment (n = 3). Error bars represent the mean +/- standard deviation of a minimum of three biological repeats. *P < 0.05, **P < 0.01, ***P < 0.005 (two-tailed, unpaired Student’s t-test). Source data are provided as a Source Data file.
