Fig. 3: TREX1 detachment from the ER and translocating to the nucleus were responsible for the elimination dysfunction of TREX1 and aberrant activation of the cytosolic DNA-sensing pathway. | Nature Communications

Fig. 3: TREX1 detachment from the ER and translocating to the nucleus were responsible for the elimination dysfunction of TREX1 and aberrant activation of the cytosolic DNA-sensing pathway.

From: Self-powered triboelectric-responsive microneedles with controllable release of optogenetically engineered extracellular vesicles for intervertebral disc degeneration repair

Fig. 3

A Upregulated “Cellular senescence” pathways and expression heatmaps between LSI and LSI-E group (n = 3 biological independent samples). B Expression heatmaps of senescence-associated secretory phenotype (SASP) genes from NP cells (n = 4 biological independent experiments). C Senescence-associated β-galactosidase (SA-β-gal) staining and quantitative analysis in NP cells (n = 4 biological independent experiments). D Representative western blotting images of cGAS, STING, and γH2A in NP cells (n = 4 biological independent experiments). E, F Representative DNA electrophoresis images (E) and quantitative analysis (F) of cytosolic DNA fragments in NP cells (n = 4 biological independent experiments). G Representative western blotting images of TREX1 in NP cells (n = 4 biological independent experiments). H, I Representative western blotting images of (H) and quantitative analysis (I) of cytosolic, nuclear, or total TREX1 protein in NP cells (n = 4 biological independent experiments). J Representative IF staining of TREX1 in NP cells, bar: 20 μm (Representative image of three independent technical experiments). K Schematic illustration of Flag-tagged TREX1 wide-type (TREX1-WT), Flag-tagged TREX1 D18N mutant (TREX1-Mut, 18th amino acid residue replaced aspartate with asparagine) and Flag-tagged N-terminus of TREX1 construct lacking a C-terminal ER-associated site (TREX1-NT, the anime-terminus from the 1st to 235th amino acids). L Representative western blotting images of Flag-tagged TREX1 variants and γH2A in NP cells after transfected with vector and Flag-tagged TREX1 variant plasmids (Representative blot of four independent technical experiments). M Quantitative analysis of western blotting results showing γH2A protein expression in NP cells (Quantification of three independent technical experiments). N, O Representative agarose gel electrophoresis images (N) and quantitative analysis (O) of cytosolic DNA fragments in NP cells (Representative blot of four independent technical experiments, and quantification of four independent technical experiments). A significant p value was determined by two-tailed unpaired t test (C, F, I) and two-tailed ANOVA (N, O). Mean ± SD are shown for (C, F, M, O). n.s. not significant.

Back to article page