Fig. 3: TLR8 is expressed by erythroid cells at early stage.
From: Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia
a Representative flow cytometry and b Wright-Giemsa staining of erythroid precursors of cord blood (CB)-derived CD34+ HSPCs. c Immunoblotting of TLR8 in erythroid cells at the indicated time points (n = 3 biologically independent experiments). d Gating strategy isolating BFU-E and CFU-E cells. e Flow cytometry of TLR8 in BFU-E and CFU-E cells (n = 3 biologically independent experiments). f Immunoblotting of TLR8 in erythroid progenitors (n = 3 biologically independent experiments). g Immunofluorescence of TLR8 in BFU-E and CFU-E cells (EEA1, early endosome antigen 1) and the quantitation (n = 39 for BFU-E, n = 43 for CFU-E from biological triplicate); scale bar = 10 μm. Data are mean ± SEM; statistical significance was determined using the unpaired two-tailed Student’s t-test for two groups comparison. ****P < 0.0001. Source data are provided as a Source Data file.
