Fig. 2: LILRB1 deficiency slows MM development and reduces tumor burden with activation of ferroptosis in vivo. | Nature Communications

Fig. 2: LILRB1 deficiency slows MM development and reduces tumor burden with activation of ferroptosis in vivo.

From: Leukocyte immunoglobulin-like receptor B1 (LILRB1) protects human multiple myeloma cells from ferroptosis by maintaining cholesterol homeostasis

Fig. 2

a–j NSG mice were injected with 2 × 106 CTR-KD or LILRB1-KD ARP-1 or MOLP-8 cells (ARP-1, male mice; MOLP-8, female mice). After 2-3 weeks of injection, MM-bearing mice were followed by bioluminescent imaging for tumor burden weekly (a, b) and summarized results of bioluminescent signals were shown (c, d). e, f Tumor burden measured as serum concentration of κ light chain or λ light chain in CTR-KD or LILRB1-KD MM-bearing mice. For (a–f), n = 5. g, h Tumor burden was measured as percentage of CD138+ MM cells in the BM in CTR-KD or LILRB1-KD MM-bearing mice (n = 3). Data are presented as mean ± SD. i, j Survival curve of CTR-KD or LILRB1-KD MM-bearing mice (i, n = 4; j, n = 5). k–m NSG mice (female) were injected with 2 × 106 CTR- or LILRB1-overexpressing MM.1 R cells, followed by monitoring of tumor burden and survival. k Tumor burden measured as serum concentration of λ light chain in CTR- or LILRB1-overexpressing MM.1R-bearing mice (n = 5). l Tumor burden measured as percentage of CD138+ MM.1 R cells in the BM in CTR- or LILRB1-overexpressing MM.1R-bearing mice (CTR, n = 2; LILRB1, n = 5). Data are presented as mean ± SD. m Survival curve of CTR- or LILRB1-overexpressing MM.1 R -bearing mice (CTR, n = 4; LILRB1, n = 5). n, o Pathway analysis of changes in RNAseq data between CTR-KD and LILRB1-KD ARP-1 cells sorted from the bone marrow of MM-bearing NSG mice. NES, normalized enrichment score. Heat map (p) and gene set enrichment analysis (q) of fatty acid metabolism- and ferroptosis-related genes in RNAseq data of CTR-KD and LILRB1-KD ARP-1 cells sorted from the bone marrow of MM-bearing NSG mice. r, s NSG mice were injected with 5 × 106 CTR-KD or LILRB1-KD ARP-1 cells (n = 9). After 7 days (r, n = 4) or 15 days (s, n = 5) of injection, lipid peroxidation of MM cells in the BM was detected. n, biological repeats, different mice samples. Data are presented as mean ± SD. Statistical significance was determined by two-tailed Student t-test. Source data are provided as a Source Data file.

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