Fig. 3: White-light activated Y6CT-NPs for in vivo angiography and monitoring of hepatic ischemia reperfusion.

a NIR-II fluorescence imaging of blood vessels of BALB/c mice in supine positions after i.v. injection of 100 μL Y6CT-NPs (300 μM) under white-light illumination activation (16.5 mW cm⁻²). Repeated for three times in independent experiments. b The cross-sectional signal-to-noise ratios and full width at half maximum was measured along the pink line. c Schematic diagram of hepatic ischemia reperfusion process. Fluorescence imaging of the liver with complete blood vessels (d) and corresponding fluorescence intensity ratio (I/I_R1) in different processes (e) of hepatic ischemia reperfusion after i.v. injection of Y6CT-NPs (300 μM, 1st injection 100 μL, 2nd injection 50 μL). Data were presented as mean ± SD derived from n = 3 independent samples per group. Fluorescence imaging of the liver with injured blood vessels (f) and corresponding fluorescence intensity ratio (I/I_R1) in different processes (g) of hepatic ischemia reperfusion after i.v. injection of Y6CT-NPs (300 μM, 1st injection 100 μL, 2nd injection 50 μL). The yellow and red ellipses denote the ischemic portion of liver, the blue rectangles denote the healthy liver, and the green ellipses denote the ___location of hepatic portal. Data were presented as mean ± SD derived from n = 3 independent samples per group. I represent the average fluorescence intensity of the analyzed area at different stages, while I_R1 indicates the initial intensity of the rectangular region after 1st injection of Y6CT-NPs. E_x = white-light illumination (16.5 mW cm⁻²). Scale bar: 5 mm.