Fig. 2: Mice lacking Rnase4 have an altered intestinal microbiota. | Nature Communications

Fig. 2: Mice lacking Rnase4 have an altered intestinal microbiota.

From: Ribonuclease 4 functions as an intestinal antimicrobial protein to maintain gut microbiota and metabolite homeostasis

Fig. 2

a High-throughput 16 S rDNA sequencing of stool bacterial DNAs from WT and Rnase4−/− mice. The y-axis represents the mean of the observed species, indicative of bacterial diversity within each group (n = 6). b Unweighted UniFrac principal-coordinate analysis of the β-diversity of microbiota composition in WT or Rnase4−/− mice (n = 6). c Quantification of unweighted UniFrac distance in (b). The boxplot represents the minimum value, first, median, and third quartiles, and maximum value. d Linear discriminant analysis effect size showing the most differentially abundant taxa of the gut microbiota between WT and Rnase4−/− mice (n = 6). e The abundance of Mucispirillum and Parasutterella in the gut microbiota of WT or Rnase4−/− mice by quantitative PCR analysis (n = 6). f Fluorescent in situ hybridization of colonic lumen sections from WT or Rnase4−/− mice showing the presence of Parasutterella (red) and Mucispirillum (cyan) in the lumen. Scale bar, 25 μm. g Analysis of the number of Parasutterella and Mucispirillum in the lumen. At least 10 sections per mouse were analyzed (n = 6). Data are presented as mean ± SEM; * p < 0.05; ** p < 0.01; *** p < 0.001 by two-tailed unpaired Student’s t-test (a, e, and g) or two-way analysis of similarities (ANOSIM) test (c).

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