Fig. 5: Multi-level analysis by Spectre shows the magnitude of polyclonal responsive activated CD8 T cell populations correlating with serum glucose and GDNF levels.
From: Non-human primate model of long-COVID identifies immune associates of hyperglycemia
a UMAP showing 18 subpopulations within CD3+ T cells in unstimulated PBMCs. Expression of selected markers is shown (b) CD8 (c) CD69 (activation), and (d–f) inflammatory markers in unstimulated PBMCs. g UMAP density plot of unstimulated cells. h UMAP showing 18 subpopulations within CD3+ T cells in PMA/I stimulated PBMCs. Expression of selected markers are shown i CD8, j CD69 (activation) and (k–m) inflammatory markers in unstimulated PBMCs. n UMAP density plot of PMA/I stimulated cells. o Heat maps showing the defining phenotypes of population 3 and 4. UMAPS are the composite of 10 unvaccinated AGMs. p Two-sided Spearman correlations between blood glucose levels in all animals and percentage of population 3 within CD3+ T cells in the PMA/I treatment. Correlation conducted between glucose and cell populations at BL and weeks 1, 4, 8, and 12. q Two-sided Spearman correlations between plasma GDNF levels in unvaccinated animals and a number of activated CD8+ T cells within CD3+ T cells in the PMA/I treatment. Source data are provided as a Source Data file.
