Fig. 7: pWNT MB show high feature conservation and can be identified by Tenascin C (TNC) staining.
A Histological, molecular, and clinical characteristics of the pWNT MB subtype (n = 19). B Differentially abundant proteins when comparing pWNT tumors to all other proteome subtypes (two-tailed, unpaired t test, p value < 0.05; log2FC > 1.5). C Scheme and representative images of digital quantification of TNC immunostainings in MB (source data file has been provided). D Significantly enhanced DHS for TNC in pWNT MB (npWNT=9) compared to all other MB subtypes (nothers=28, two-tailed, unpaired t test, p < 0.0001, data are presented as mean values ± SD). E Protein abundance for TNC in pWNT MBs (npWNT = 19) compared to all other MB subtypes (nothers=148, two-tailed, unpaired t test, p < 0.0001, data are presented as mean values ± SD). F TNC gene expression in WNT MBs and other MB subtypes in a published dataset of MB (nWNT = 70, nnonWNT = 693, two-tailed, unpaired t test, p < 0.001, data are presented as mean values ± SD)5. G Average DNA methylation at CpG sites of the TNC gene (mean value for npWNT = 8 CpG sites shown, two-tailed, unpaired t test, p = n.s., data presented as mean values ± SD). H MCL clustering of eEnriched gene sets, comparing pWNT to all other subtypes in GSEA. I Heatmaps showing mean protein abundance in MB subtypes for hallmark genesets specifically enriched in pWNT MB (GSEA differential expression analysis normalized enrichment score (NES), NESGlycan = 2.2, pGlycan = <0.001; NESEMP = 1.7, pEMP = 0.02). J CNV plots of pWNT MBs (n = 8) were calculated from either DNA methylation or proteome data with Pearson correlation between both omic types (r = 0.37). n represents biologically independent human samples. For immunostaining, each sample was stained once. NS = not significant.
