Fig. 1: RxLR23 effector is required for virulence of P. capsici.

A, C Virulence of RxLR23 knockout mutants acts on C. annuum and N. benthamiana leaves. T-7 and T-10 are knockout mutants. T-13 is an unknockout mutant. WT is SD33 strain. Leaves of C. annuum and N. benthamiana were inoculated with P. capsici zoospores from T-7, T-10, T-13, or WT strain. Images were taken under UV light for C. annuum leaves at 72 hpi and for N. benthamiana leaves at 48 hpi (n = 30 samples). B, D Cell death was scored by trypan blue staining on C. annuum and N. benthamiana leaves, respectively (n = 30 samples). E Lesion spread diameter of 30 leaves infected by mutants relative to WT in C. annuum or N. benthamiana leaves. Values are presented as mean ± SD, n = 30 samples (ANOVA, *p < 0.05, ***p < 0.001). F qPCR was used to measure the relative biomass on the ratios of P. capsici to C. annuum or N. benthamiana DNA at 48 hpi. Pcβ-actin, CaEIF5A2, and NbEF1α were identified as the most suitable reference genes for normalization. The degree of relative biomass of infected C. annuum or N. benthamiana leaves with WT strain was assigned to value 1.0. The data graphs present the means and error bars represent ± S.D from three independent experiments (ANOVA, ***p < 0.001). These experiments (A–E) were repeated at least three times. Source data are provided as a Source Data file.