Fig. 6: Enhanced potency of HDR-boosting modular donors with HDRobust or M3814.
From: Enhancing homology-directed repair efficiency with HDR-boosting modular ssDNA donor
a Genome editing efficiencies at four endogenous loci in HEK 293T cells introduced by the indicated donors, along with transient end-joining inhibition by HDRobust (M3814 + POLQ siRNA mix) or M3814. b Genome editing efficiencies at five endogenous loci in K562 cells introduced by the indicated donors, with or without treatment by M3814. Electroporation was carried out using a 20 µl mixture, containing 2 × 105 cells, 50.4 pmol Cas9 nuclease, 64 pmol gRNA and 40 pmol ssDNA donors. When applicable, we added POLQ siRNA mix containing 32 pmol of POLQ siRNA predesigned pool (siRNAs 485, 1390, 1397 and 2460) and 64 pmol of POLQ siRNA 765. For transient NHEJ inhibition, 2 µM M3814 was added for two days after electroporation, and editing efficiency was measured five days after electroporation. Values reflect n = 2 (a, b) independent electroporation replicates. The sequences of all gRNAs, ssDNA donors and POLQ siRNA used are shown in Supplementary Data 1, 2 and 6. Source data are provided as a Source Data file.
