Fig. 7: Arabidopsis prot2 leaves have elevated levels of apoplastic proline and are more susceptible to P. syringae infection. | Nature Communications

Fig. 7: Arabidopsis prot2 leaves have elevated levels of apoplastic proline and are more susceptible to P. syringae infection.

From: Transporter-mediated depletion of extracellular proline directly contributes to plant pattern-triggered immunity against a bacterial pathogen

Fig. 7

a GC-MS analysis of proline in apoplastic wash fluid (AWF) from Col-0 and prot2-3 leaves. Graphed are means ± SE of normalized peak area for proline, n = 4 from four independent experiments. Col-0 and prot2-3 leaves were infiltrated with P. syringae DC3000 harboring either (b) putApromoter:gfp or (c) hrpLpromoter:gfp, or an empty:gfp plasmid. Graphed are means ± SE of normalized GFP fluorescence of leaf tissue six hours post-infection, n = 12. d 2 x 106 cfu/mL of P. syringae DC3000 was infiltrated into Col-0 and prot2-3 leaves. Leaf bacteria were enumerated 3 days post-infection by serial dilution plating of leaf extracts. Graphed are log transformed means ± SE of colony-forming units (cfu), n = 3 for day 0 samples, n = 4 for day 3 samples. e 2 x 106 cfu/mL of P. syringae DC3000 or DC3000ΔputA was infiltrated into Col-0 and prot2-3 leaves. Leaf bacteria were enumerated 3 days post-infection by serial dilution plating of leaf extracts. Graphed are log transformed means ± SE of colony-forming units (cfu), n = 12. f Proline levels in AWF from Col-0 and prot2-3/prot3-2 leaves treated for six hours with 100 nM flg22 or a mock treatment. Graphed are means ± SE of normalized peak area for proline, n = 4. g P. syringae DC3000 growth in Col-0 or prot2-3 plants pre-treated for six hours with 100 nM flg22 or a mock treatment. Graphed are log-transformed means ± SE of cfu isolated from infected tissue 3 days post-infection, n = 10. h 13C-proline uptake in prot2-3 leaves treated for six hours with 100 nM flg22 or a mock treatment. Graphed are means ± SE of normalized peak area for proline, n = 3. Asterisks in panels a–d, f and h denote statistical significance based on two-sided t-test, *p < 0.05. **p < 0.01. Lower case letters in panels e and g denote significance groupings based upon ANOVA with Tukey’s HSD, p < 0.05. Data in panels b–d are representative of three independent experiments. Data in panels e–h were pooled from three independent experiments.

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