Fig. 1: Antibiotic killing of non-growing B. subtilis cells.
From: Membrane depolarization kills dormant Bacillus subtilis cells by generating a lethal dose of ROS
Survival curves of exponentially growing (a and c), and stationary (dormant) phase (b and d) B. subtilis cells incubated with different antibiotics. Cells were treated with either 20 µg/ml vancomycin (van), 2 µg/ml ciprofloxacin (cipro), 10 µg/ml kanamycin (kan) or 100 µM valinomycin (val). 1 % DMSO (w/w) was used as control. B. subtilis strains contained a deletion in spoIIE and were unable to sporulate. Percent survival was determined through plating serial dilutions and viable count measurements every 2 h. Data shown reflect mean ± SD (standard deviation) of three biological replicates.
