Fig. 6: Localization of cytochrome bc1 and cytochrome-c oxidase subunits and cytochrome c550 fused to GFP.
From: Membrane depolarization kills dormant Bacillus subtilis cells by generating a lethal dose of ROS
a Cellular localization of GFP tagged cytochrome bc1 subunits QcrA (Rieske protein), QcrB and QcrC, cytochrome-c oxidase subunits CtaC and CtaD, and cytochrome c550 in stationary phase cells incubated with 100 μM valinomycin for 1, 2 and 4 h. The experiment was repeated three times with similar results. b Same as a except that the cells were sampled from the exponential phase and treated with 100 μM valinomycin for only 10 to 30 min. Arrows indicate clustering of the GFP-QcrA signal. Scale bars represent 3 µm. Large field images with several cells are shown in Fig. S13 and S14. The experiment was repeated three times with similar results.
