Fig. 2: Global kinetic analysis of the TRXL data of AsLOV2 I427V. | Nature Communications

Fig. 2: Global kinetic analysis of the TRXL data of AsLOV2 I427V.

From: Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein

Fig. 2

a Experimental (black) and calculated (red) difference scattering curves. b The top panel represents the sequential kinetic model of AsLOV2 determined in this study. The lower panel shows the SADSs obtained through KCA analysis. SADS1 (black), SADS2 (red), and SADS3 (blue) correspond to I1, I2, and P (photoproduct) of the sequential kinetic model, respectively. The static difference curve (qΔSstatic, light state – dark state, gray), normalized to SADS3, is also shown. c Time-resolved changes in the population of transient species (I1 (black), I2 (red), and P (blue)). Since the protein exhibits dimer conformation in the P state, the maximum population of P is 0.5. This value is half those of the other states (G, I1, and I2), which exhibit monomer conformations.

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