Fig. 4: Bio-layer interferometry binding assay for CR3022 and anti-5T4 UdAbs candidates.
From: Pretrainable geometric graph neural network for antibody affinity maturation
a, d Illustration of the experiment protocol with (a) Ni-NTA and d SA biosensors. b, e The binding kinetics of different CR3022 (b) and anti-5T4 UdAbs (e) candidates. The starting concentration for each antibody was 300 nM. The data were determined by fitting curves to a global 1:1 binding model. The KD (equilibrium dissociation constant) values, annotated on each plot, were determined with R2 values of greater than 98% confidence level. c, f The kon (association rate constant), koff (dissociation rate constant), and KD values of mutant and wild-type CR3022 antibodies (c) and anti-5T4 UdAbs (f).
