Fig. 2: PHF6 is required for maintenance of H3K14 and H3K27 acetylation.
From: PHF6 cooperates with SWI/SNF complexes to facilitate transcriptional progression
a Volcano plot displaying histone post-translational modification changes as determined by mass spectrometry of histones extracted from G401 shPHF6 versus shCTRL cells. Two-tailed homoscedastic Student’s t-test was performed. Data are represented from mean of three biological replicates. b Western blots of histone modifications in histone extracts prepared from RT cells 72 hr post selection with shCTRL or shPHF6 knockdown (n = 3 independent biological replicates). c Western blots of histone modifications in G401 cells re-expressing inducible exogenous PHF6 (72 h) after knockdown of endogenous PHF6 (n = 2 independent biological replicates). d Metagene plots of H3K14ac, H3K27ac centered at the TSS ± 2 kb in shCTRL and shPHF6 G401 cells (n = 3 independent biological replicates). e Re-expression of doxycycline-inducible exogenous PHF6 (72 h) in DND41 cells that naturally lack PHF6 expression (n = 2 independent biological replicates). Source data are provided as a Source Data file.
