Fig. 10: Cadmium stress-induced NoSB formation in a nosr-1-independent manner.

A DIC and fluorescence microscopy images of hypodermal and intestine nuclei after cadmium treatment in the indicated animals expressing the GFP::NUMR-1. Arrows indicate NoSB (scale bar, 5 μm). All images were taken by the Leica THUNDER imaging System. B Quantification of NoSB in hypodermic cells and intestine cells from (A). Every dot indicates the percentage of hypodermis or intestine cells containing NoSB in each worm. Red dots indicate wild-type animals, blue dots nosr-1(ust303) mutants, green dots nosr-1(ust308) mutants. n, the number of animals tested. The horizontal line represents the median value, the lower and upper quartiles represent the 25th and 75th percentile, and the whiskers show the maximum and minimum values. A two-tailed t-test was performed to determine statistical significance. C A working model of NoSB formation under nucleolar stress in C. elegans. Source data are provided as a Source Data file.