Fig. 3: NoSB is a distinct subnuclear organelle. | Nature Communications

Fig. 3: NoSB is a distinct subnuclear organelle.

From: Nucleolar stress induces nucleolar stress body formation via the NOSR-1/NUMR-1 axis in Caenorhabditis elegans

Fig. 3

A List of marker genes of subnuclear organelles and their C. elegans homologs. B–D DIC and fluorescence microscopy images of hypodermic cells of L4 animals expressing TagRFP::DAO-5, RBD-1::mCherry, RRP-8::GFP and HSF-1::GFP after exos-9 knockdown (Scale bar, 5 μm). Yellow arrows indicate NoSB. Three times were repeated independently with similar results. E Schematic diagram of the ___domain structure and predicted intrinsically disordered regions of NUCL-1 and RRP-8. F DIC and fluorescence microscopy images of nuclei with the indicated transgenes after exos-9 knockdown (scale bar, 5 μm). Three times were repeated independently with similar results. All images were taken by the Leica THUNDER imaging System.

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