Fig. 6: Nucleolar stresses activate nosr-1 expression.

A Volcano plot comparing the transcriptomes of the indicated animals. Volcano plot showing log₂ (fold change) of gene product-annotated transcript abundances in stressed vs control-animals (x axis) and −log₁₀ (P value) (y axis), calculated using an exact test in edgeR. The dashed line represents the significance threshold. The expression of nosr-1 is highlighted. B Fluorescence microscopy images of nosr-1_p::NLS::GFP(ustIS285) after actinomycin D (abbreviated as Act D) treatment. C Fluorescence microscope images of nosr-1_p::NLS::GFP(ustIS285) in the indicated animals. D Fluorescence microscope images of nosr-1p::NLS::GFP after knocking down the indicated genes by RNAi (scale bar, 20 μm). All worms were imaged at the L4 stage and all images are representative of more than ten animals (B–D). All images were taken by the Leica THUNDER imaging System. E, F Relative mRNA levels of nosr-1 measured by quantitative real-time PCR in the indicated animals. Data were presented as the means ± SD of three and four biological replicates, respectively. A two-tailed t-test was performed to determine statistical significance. Source data are provided as a Source Data file.