Fig. 2: Three-way association among cg03609847, PIGG gene expression, and ACS risk. | Nature Communications

Fig. 2: Three-way association among cg03609847, PIGG gene expression, and ACS risk.

From: Genome-wide DNA methylation profiling in blood reveals epigenetic signature of incident acute coronary syndrome

Fig. 2

a The schematic diagram depicting association directions between DNAm, mRNA expression, and ACS risk. b The correlation between DNAm of cg03609847 and PIGG gene expression levels in leukocytes of 156 healthy participants was examined by Pearson correlation test. c The comparison of PIGG gene expression levels in leukocytes between 12 pairs of ACS cases and matched controls was analyzed using the “limma” package. d The comparison of PIGG gene expression levels in 32 pairs of normal carotid tissue and carotid atheroma plaques was performed by GEO2R online tool. e The comparison of PIGG gene expression in 13 early atherosclerotic carotid artery segments and 16 advanced atherosclerotic carotid artery segments was performed by GEO2R online tool. All P values were two-sided. All box plots show median value, IQR, up to 1.5 IQR (whiskers). RNA expression was quantified by TPM across all genes, followed by log2(TPM + 1) transformation for subsequent analysis. ACS acute coronary syndrome, DNAm DNA methylation, IQR interquartile range, TPM transcripts per million.

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