Fig. 7: PSFL77 is capable of causing hypersensitivity to pain in P2rx3+/+, but not in P2rx3−/−, mice. | Nature Communications

Fig. 7: PSFL77 is capable of causing hypersensitivity to pain in P2rx3+/+, but not in P2rx3−/−, mice.

From: Finely ordered intracellular ___domain harbors an allosteric site to modulate physiopathological function of P2X3 receptors

Fig. 7

A An illustration of the von Frey test procedure in mice. B Effects of PSFL77 on the mechanical withdrawal threshold in P2rx3+/+ and P2rx3−/− mice. Data are presented as mean ± S.E.M. (n = 6 (Vehicle P2rx3+/+ and AF-353 P2rx3+/+), 7 (PSFL77 P2rx3−/−), or 10 (PSFL77 P2rx3+/+). * P < 0.05 and ** P < 0.01 vs. vehicle P2rx3+/+, two-way ANOVA with Dunnett’s multiple comparisons test, P = 0.0039 (10 min), 0.0196 (20 min) for AF-353 P2rx3+/+, P < 0.0001 (10, 20, 30, 45, 60, 75, 90, 105 and 120 min), 0.0004 (150 min), and 0.0446 (180 min) for PSFL77 P2rx3+/+. C Representative images depicting cells expressing rP2X2-pIRES-eGFP and rP2X3-pIRES-mCherry, with pseudo-colors assigned to eGFP (green) and mCherry (red). D, E Representative current traces (B) and pooled data (C) showing the effect of PSFL77 (100 μM) on rP2X2/3 heterotrimers (mean ± S.E.M., n = 4 (rP2X2/3), 5 (rP2X2) or 8 (rP2X3). * P = 0.0121 and ** P = 0.0008 vs. rP2X2WT, two-side unpaired t test).

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