Fig. 3: HDX-MS analysis of conformational changes in parkin upon pUb and BIO-2007817 binding. | Nature Communications

Fig. 3: HDX-MS analysis of conformational changes in parkin upon pUb and BIO-2007817 binding.

From: Activation of parkin by a molecular glue

Fig. 3

a Changes in solvent exposure of the parkin complex upon addition of pUb at four time points (15 s, 1 min, 10 min, 60 min) and the sum of the four time points (gray bars). The largest changes occur in the Ubl ___domain, which is released by pUb binding, and RING1, which is the site of pUb binding. b Changes in solvent exposure upon addition of BIO-2007817 in the presence of pUb. The largest changes occur in the Rcat ___domain. c, d Addition of BIO-2007817 does not change solvent exposure in the absence of pUb or in the parkin K211N mutant. e HDX-MS analysis of individual peptides from the parkin Rcat ___domain show solvent exposure in the presence of BIO-2007817 but not the inactive diastereomer BIO-2007818. f Mapping of increased (red) and decreased (blue) exchange upon BIO-2007817 addition to parkin:pUb (b).

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