Fig. 8: Gs-derived Gβγ drives the Gs-dependent, cAMP-independent Ca²⁺ release pathway.

a Representative Ca²⁺ traces and their quantification evoked in HEK-∆_fPLCβ1–4 cells transiently transfected to re-express PLCβ3 in the absence and presence of the Gβγ-scavenger masGRK3ct. Cells were primed with CCh at t = 20 s followed by addition of Iso at t = 140 s. b Same experimental setup as in (a) using HEK293-wt cells and 1 µM CCh as the first stimulus. The concentration-response curves derived from the mean net peak responses are divided into a high-potency and a low-potency component, reflecting Gα_s-cAMP “α” and Gs-βγ “βγ” contribution, respectively. Bar graphs represent the fractional distribution of high- and low-potency Iso-Ca²⁺ and its alteration with co-expressed masGRK3ct. Representative traces are mean + SEM, and data points in concentration-response curves are mean ± SEM of n biologically independent experiments (a: vector n = 6, masGRK3ct n = 5; b: n = 5), each performed in duplicate. Source data are provided as a Source Data file.