Fig. 5: Baculovirus transduction in mouse liver is dependent on NPC1.

a Investigation of the tissue tropism of Bac-EGFP in wild-type mice at 72 h post intravenous injection, as determined by EGFP expression at protein (n = 3). The fluorescence images are acquired using confocal microscopy on flash-frozen tissues that are fixed with optimal cutting temperature compound (OCT). b RT-qPCR quantification of NPC1 mRNA in the lysate of liver, kidney, lung, brain, and spleen in wild-type and Npc1^−/+ mice. The p-value between WT and Npc1^−/+ in spleen groups is 1e-8. RT-qPCR quantification of EGFP mRNA in the lysate of liver (c) and other tissues including kidney, lung, brain, and spleen (d) in wild-type and heterozygous Npc1^−/+ knockout mice at 72 h post-Bac-EGFP injection. The EGFP mRNA in the lysates of kidney, lung, brain, and spleen (d) in heterozygous Npc1^−/+ knockout mice is below the limit of detection. For (b–d), β-actin is used as an internal control. The data in this figure are from independent biological replicates. The significant difference is analyzed by two-tailed unpaired Student’s t-test. For (b and d), Data are presented as mean ± SD of biological replicates (n = 5 for (b) and = 3 for (d)). For c, Data are collected from nine biological replicates (n = 9). The thick horizontal dashed line represents the median and thin horizontal dashed line indicates interquartile range between 25th and 75th percentile. Source data are provided as a Source Data file.