Table 1 In vitro kinetic characterization of AGE and mutant activities
Enzymes | Km (μM) | kcat (s−1) | Vmax (μM min−1) | kcat/Km (s–1 M–1) |
---|---|---|---|---|
AGE | 49.2 | 2.3 | 0.4 | 46,266.9 |
AGEOMeY | 1448.9 | 124.7 | 24.4 | 86,078.9 |
Enzymes | Km (μM) | kcat (s−1) | Vmax (μM min−1) | kcat/Km (s–1 M–1) |
---|---|---|---|---|
AGE | 49.2 | 2.3 | 0.4 | 46,266.9 |
AGEOMeY | 1448.9 | 124.7 | 24.4 | 86,078.9 |