Fig. 4: Reduced repertoire of distinct neuronal ensembles during sleep after sphere-rearing.
a Examples of spike count correlations between cell pairs across 200 frames for 3 pyramidal cells during Pre-Day1Run1 sleep in cuboid- (left) and sphere-reared (right) rats. Top: reference cell for correlations. Total spike counts are given in brackets next to cell ID. b Cumulative distributions of correlations of spike counts between pyramidal cell pairs across sleep frames. Note higher values in sphere- vs. cuboid-reared rats (p = 2.7 × 10−5, two-sided rank-sum test). c Shuffle-normalized correlations of cell pairs are higher in sphere- vs. cuboid-reared rats (p = 7.3 × 10−6, two-sided rank-sum test). Inset: proportion of significantly correlated cell pairs (>95th percentile of shuffles) is higher in sphere- vs. cuboid-reared rats (p = 1.9 × 10−7, two-sided Z-test for 2 proportions). d Similarity scores (p = 3.0 × 10−301, two-sided rank-sum test) and proportions of correlated Pre-Day1Run1 sleep frame pairs (inset; p = 0, one-sided Binomial tests vs. chance for both groups) vs. chance. Note higher scores and proportions in sphere-reared vs. control rats (p = 1.9 × 10−238, two-sided Z-test for 2 proportions). e Examples of clustered Pre-Day1Run1 sleep frames with correlated activity in cuboid- (top) and sphere-reared (bottom) rats. Colorbar: color-coded clusters and cluster demarcation. Curves to the right of cluster panels: Proportions of frames from each cluster that are significantly correlated with the future run trajectory. f Number of clusters during Day1 sleep sessions (cuboid > sphere, p = 0.0051, two-sided rank-sum test). N = 10 (5 rats/group; 2 sleep sessions/rat). g Distributions of Pre-Day1Run1 sleep frame-pair correlations (within>across clusters, p = 0; sphere vs. cuboid, within or across clusters: p = 0, two-sided exact permutation tests). b–d, g N = 5 rats/group. ***p < 0.005. **p < 0.01. Source data are provided as a Source Data file.
