Fig. 6: Enrichment analysis.

Competitive phylum enrichment analysis, contrasting the phyla Bacillota and Bacteroidota. Enrichment is assessed using camera PR and t-statistics to rank species based on their differential expression. These are used to determine statistically significant variations in the aggregate mean rank order for the entire phylum between different iron and placebo. In the barcode plots, individual species within a phylum are represented by vertical bars; species that are up-regulated under placebo conditions appear on the left, while those up-regulated in response to the intervention are on the right. The line above represents the distribution of t-statistics, highlighting enrichment of species within the phyla. A Bacteroidota (placebo-iron), (B) Bacillota (placebo-iron), (C) Bacteroidota (placebo-MNPs) and (D) Bacillota (placebo-MNPs) (Shotgun metagenomic data).