Fig. 4: Cryo-EM structure of FMDV dissociated pentamer. | Nature Communications

Fig. 4: Cryo-EM structure of FMDV dissociated pentamer.

From: Foot-and-mouth disease virus antigenic landscape and reduced immunogenicity elucidated in atomic detail

Fig. 4

a Cryo-EM structure of FMDV dissociated pentamer from outside (left) and inside (left middle). The FMDV viral proteins are colored as shown in the legend, while 146S indicates a pentamer that is present in a 146S particle. Electron density maps for C- or N-terminus of VP proteins of the dissociated pentamer (right). b Comparison of the structure of a dissociated pentamer (color coded according to panel a) with a superimposed 146S pentamer (white colored) is shown in front view (left), side view (upper right) and cross-section of side view (lower right) on left of the panel. The disordered and thus unresolved N-termini of VP1 and N/C-termini VP2 and the loss of VP4 of dissociated pentamer are displayed on the right. The viral proteins are color coded as panel a. c, d Structural comparison between 146S and 12S at the protomer level (c) and local conformation change within a protomer (d). e Total FMDV-specific antibody titers (left) and the NAb titers (right) observed after immunizing two groups of pigs with equimolar doses of 146S and 12S at days post vaccination 0, 7, 14, 21, 28. Data are presented as mean values +/- SD of n = 6 biologically independent samples for each group are shown. Source data are provided as a Source Data file.

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