Fig. 8: Enhanced radioimmunotherapy with RMn-NBs in B16F10-OVA tumor models.
a Tumor growth curves of B16F10-OVA tumor-bearing C57BL/6 mice treated with Saline, Saline + RT, RMn-NBs + RT, RMn-NBs + RT + αCD8a and RMn-NBs + RT + αPD-L1. Treatments are performed with 3 fractions on days 0, 2, and 4 (black arrow). αPD-L1 and αCD8a are intraperitoneally injected respectively after RT for 6 h (red arrow, n = 6 mice). b Tumor weights from mice with various treatments (n = 6 mice). c F4/80+ and CD11b+ macrophages in B16F10-OVA tumor-bearing C57BL/6 mice with different treatments detected by FCM (n = 6 mice). d FCM analysis of CD11c+ H2Kb-SIINFEKL+ DCs in tumor-draining lymph nodes from mice treated with Saline, Saline + RT, RMn-NBs + RT, RMn-NBs + RT + αCD8a and RMn-NBs + RT + αPD-L1 (n = 6 mice). e Mature DCs (CD80+ and CD86+ gated on CD11c+) in B16F10-OVA tumor-bearing C57BL/6 mice with different treatments detected by FCM (n = 6 mice). f The percentages of CD3+ CD4+ T cells infiltrated within tumor tissues detected by FCM (n = 6 mice). g The percentages of CD3+ CD8+ T cells infiltrated within tumor tissues detected by FCM (n = 6 mice). h Percentages of effector memory T cells (TEM) in the spleen analyzed by FCM. The spleens in different groups are collected on day 10 (n = 6 mice). i The quantification of antigen-specific T cells by IFN-γ ELISpot (n = 3 mice). j The representative images of antigen-specific T cells by IFN-γ ELISpot. This experiment is repeated twice independently with similar results. All data are shown as mean ± SD. Statistical analysis is performed using two-tailed Student’s t-test for comparing two groups, and one-way ANOVA analysis of variance for multiple groups. p > 0.05 represent nonsignificance, while p < 0.05 indicate statistical significance. Source data are provided as a Source Data file.
