Fig. 8: The human immune response during craniotomy and prosthetic joint infection (PJI) is transcriptionally diverse.
From: Tissue niche influences immune and metabolic profiles to Staphylococcus aureus biofilm infection
Single cells from blood and tissue samples of three PJI and four craniotomy infection (Cranio) patients were analyzed by scRNA-seq. A, B Cell identity separated by C, D infection type and E, F specimen source was overlayed on the integrated UMAPs (A, C, E) and (B, D, F) summarized in aggregate to describe the dataset. G PMNs were segmented from the larger dataset, H assessed for infection site composition, and I galea and PJI tissue PMNs were differentially analyzed by IPA to identify pathways enriched in each tissue milieu. A statistical pipeline in IPA was used to identify significant pathway differences, which relies on the right-tailed Fisher’s exact test. HSC hematopoietic stem cell. Source data are provided as a Source Data file.
