Fig. 6: Increased GABAergic signaling in Mtb infected GID/CTLH knockout macrophages. | Nature Communications

Fig. 6: Increased GABAergic signaling in Mtb infected GID/CTLH knockout macrophages.

From: Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth

Fig. 6

A Representative confocal images of Mtb infected anti-GABA stained BMDMhs. Scramble, GID8−/−, MAEA−/− and WDR26−/− BMDMhs were infected with the Mtb Erdman hsp60::GFP strain at MOI 4 for 24 hours. Fixed and permeabilized cells were then stained with anti-GABA (red) for confocal imaging. B Quantification of mean fluorescence intensities (MFI) of GABA staining in (A) and Fig. S10A. MFI was quantified in at least 50 cells for both scramble and knockouts; n = 50 cells. *P < 0.05; ***P < 0.001; ****P < 0.0001, two-way ANOVA alongside Dunnett’s multiple comparison test. Data are presented as mean values ± SD. Source data are provided as a Source Data file.

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