Fig. 2: LC-MS/MS chromatograms of CYP reconstitution assays using various sterol substrates. | Nature Communications

Fig. 2: LC-MS/MS chromatograms of CYP reconstitution assays using various sterol substrates.

From: CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles

Fig. 2

Recombinant CYP5122A1 and CYP51 were reconstituted with (A) lanosterol, (B) 4,14-DMZ, (C) FF-MAS, (D) T-MAS, (E) zymosterol, and (F) 7-dehydrodesmosterol as the sterol substrates. The peaks of the internal standard cholesterol-d7 (black), substrates (blue), hydroxy metabolites (orange), aldehyde metabolites (cyan), carboxylate/formyloxy metabolites (purple), and 14-demethylated products (red) were indicated in the chromatograms. Peaks with asterisk were detected in both incubations with or without NADPH and hence considered as background signals.

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