Table 3 Effects of different CYP inhibitor treatments on the sterol profile of LV82 promastigotes

From: CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles

Sterol Group

Sterol name

20 μM clotrimazole vs. DMSO

50 μM voriconazole vs. DMSO

4- and/or 14-methylated sterols

Lanosterol

2.7 ± 0.9**

1.6 ± 0.2

4CH2OH-LS

2.3 ± 0.5

0.83 ± 0.09

4,14-Dimethylzymosterol

43 ± 18***

17 ± 2**

14-Methylzymosterol

2.3 ± 0.4**

8.0 ± 1.0**

14-Methylfecosterol

Accumulateda,***

Accumulateda,***

4-Methylzymosterolb

0.025 ± 0.013***

0.18 ± 0.06**

FF-MAS

Not detected

Not detected

T-MAS

Not detected

Not detected

4,14-demethylated sterols

Zymosterolc

0.018 ± 0.006**

0.067 ± 0.034**

Fecosterolb

0.025 ± 0.013***

0.18 ± 0.06**

Episterolb

0.025 ± 0.013***

0.18 ± 0.06**

Cholesta-7,24-dienolc

0.018 ± 0.006**

0.062 ± 0.030**

5-dehydro sterols

7-Dehydrodesmosterol

0d,**

0d,**

5-Dehydroepisterol

0.20 ± 0.04**

0.078 ± 0.043***

Ergosta-5,7-dienol

0.79 ± 0.26

0.39 ± 0.11**

Ergostatetraenol

0 d,**

0.086 ± 0.027**

Ergosterol

0.26 ± 0.07**

0.30 ± 0.08***

Exogenous

Cholesterol

1.1 ± 0.2

0.88 ± 0.07

  1. The sterol levels in inhibitor-treated parasites were normalized to the corresponding sterol levels in DMSO-treated parasites. The values represent mean ± standard deviation. Each ratio is an average of three individual experiments conducted on separate days. Each experiment had biological triplicates. Source data are provided as a Source Data file.
  2. aThis sterol was detected in inhibitor-treated samples but not in vehicle-treated samples
  3. bEpisterol, fecosterol and 4-methylzymosterol could not be chromatographically separated.
  4. cZymosterol and Cholesta-7,24-dienol could not be chromatographically separated.
  5. dThis sterol was detected in vehicle-treated samples but not in inhibitor-treated samples.
  6. **, *** unpaired two-tailed t-test (**P < 0.01; ***P < 0.001).
Back to article page