Fig. 3: FTOrs9939609-A causes more rapid proliferation and senescence. | Nature Communications

Fig. 3: FTOrs9939609-A causes more rapid proliferation and senescence.

From: An obesogenic FTO allele causes accelerated development, growth and insulin resistance in human skeletal muscle cells

Fig. 3

a Representative images of FTOrs9939609-TT-myoblasts and FTOrs9939609-A-myoblasts that were automatically counted for Hoechst 33342+ cell numbers daily for 72 h using the Operetta high-content screening microscope. Scale bars, 100 μm. b Quantification of fold change in myoblasts cell numbers, n = 3 biologically independent samples. c Representative images of senescent FTOrs9939609-TT-myoblasts and FTOrs9939609-A-myoblasts by passage 16. Senescent cells were stained for β-galactosidase (SA-βgal). Scale bars, 100 μm. d Quantification of percentage of senescent FTOrs9939609-TT-myoblasts and FTOrs9939609-A-myoblasts (FTO-TT vs. FTO-A, P = 0.00250), n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. P values were calculated by two-tailed unpaired t-test. *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.

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