Fig. 5: NbHSP90s interact with NbNPR3, a negative regulator of SA signaling and both NbHSP90s and NbNPR3 are required for the suppression of SA signaling by TbCSB βC1. | Nature Communications

Fig. 5: NbHSP90s interact with NbNPR3, a negative regulator of SA signaling and both NbHSP90s and NbNPR3 are required for the suppression of SA signaling by TbCSB βC1.

From: Viral proteins resolve the virus-vector conundrum during hemipteran-mediated transmission by subverting salicylic acid signaling pathway

Fig. 5

a Interactions between NbHSP90s and NbNPR3 in the yeast two-hybrid assay. b, c Interaction between NbNPR3 and NbHSP90-2 (b) or NbHSP90-10 (c) in the co-IP assay. NbNPR3-Flag and NbHSP90s-HA were co-expressed in plants, and NbNPR3-Flag+GFP-HA served as a negative control. d, e Interactions between NbNPR3 and NbNPR3 in the co-IP (d) and yeast two-hybrid (e) assay. f, g Relative mRNA level of PR1a (f) and PR2 (g) in N. benthamiana plants that were first inoculated with TRV-GFP or TRV-NbNPR3 and then sprayed with ethanol (control) or SA. h Quantity of TbCSV in N. benthamiana plants that were first inoculated with TbCSV+TbCSB+TRV-GFP or TbCSV+TbCSB+TRV-NbNPR3 and then sprayed with ethanol (control) or SA. i, j Relative mRNA level of PR1a (i) and PR2 (j) in wild type and TbCSB βC1-transgenic N. benthamiana plants that were first inoculated with TRV-GFP or TRV-NbHSP90-2 and then sprayed with SA. k, l Relative mRNA level of PR1a (k) and PR2 (l) in wild type and TbCSB βC1-transgenic N. benthamiana plants that were first inoculated with TRV-GFP or TRV-NbNPR3 and then sprayed with SA. N = 5–7 samples (2–3 plants per sample) for (f), 4–7 samples (2–3 plants per sample) for (g), 15–18 plants for (h), 7–8 samples (2–3 plants per sample) for (i, j), 5–8 samples (2–3 plants per sample) for (k, l). Data are mean ± SEM. n. s. stands for no significant difference, *P < 0.05, and **P < 0.01 (two-sided Student’s t test for f–l). Source data and the exact P-values are provided in Source Data file.

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