Fig. 1: Lack of WAKL4 enhances Cd sensitivity. | Nature Communications

Fig. 1: Lack of WAKL4 enhances Cd sensitivity.

From: The Arabidopsis receptor-like kinase WAKL4 limits cadmium uptake via phosphorylation and degradation of NRAMP1 transporter

Fig. 1

a Phenotypes of wakl4 CRISPR/Cas9 mutants in response to Cd treatment. 5-day-old seedlings were transferred onto 1/2MS medium supplemented with (+Cd) or without 75 μM CdCl2 (-Cd). Pictures were taken 7 d after the transfer. Scale bar, 1 cm. b–c Statistical analysis of primary root length (b) (n = 24 plants) and fresh weight (c) (n = 24 independent shoot pools, 6 plants/pool) of seedlings shown in (a). Centerlines in the boxplots show the medians, and box limits indicate the 25th and 75th percentile. The whiskers go down to the smallest value and up to the largest. d Content of chlorophyll A and B shown in (a) (n = 20 independent shoot pools). e ICP-MS analysis of shoot Cd concentrations shown in hydroponics (n = 8 independent pools). Data are presented as mean values ± SD (d, e). Three independent repeats were done with similar results. Data were analyzed by ordinary one-way ANOVA (b-d) or two-tailed unpaired t-test (e) (**P < 0.01, ***P < 0.001, ****P < 0.0001).

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