Fig. 3: Non-cancer cell infections promote DC activation and migration to the TdLN.

a OVA-specific CD8⁺ T cell count in M3-9-M^Res1/OVA tumours 6 days post VSV^∆M51 (n = 10 mice/group (control), n = 11 mice/group (VSV), compiled from 2 independent experiments, two-tailed, unpaired t test). b Overall survival of M3-9-M^Res1-bearing mice treated with PBS/VSV^∆M51 ± FTY-720 (n = 17-22 mice/group, compiled from 3 independent experiments, log-rank Mantel-Cox test). c Tumour growth of mice in b (n = 5 mice/group (control), n = 6 mice/group (VSV), representative of 3 independent experiments). d Frequency of total DCs (left) and CD103⁺ DCs (right) of CD45⁺ cells in the TdLN of M3-9-M^Res1-bearing mice 24 h post PBS/VSV^∆M51 (n = 14 mice/group (control), n = 23 mice/group (VSV), compiled from 4 independent experiments, two-tailed, unpaired t test). e Expression of CD80 (left) and CD86 (right) on TdLN DCs of mice treated as in d (n = 3–5 mice/group, representative of 4 independent experiments, multiple two-tailed unpaired t tests with Holm-Sidak post-test). f–h Count (f) and expression of CD80 (g; left), CD86 (g; right) or CCR7 (h) on DCs in tumours of mice treated as in d (for f, h: n = 7–10 mice/group, compiled from 2 independent experiments; for g: n = 3–5 mice/group, representative of 2 independent experiments; two-tailed, unpaired t test). i, j Count of mCherry⁺ total DCs (left) and mCherry⁺CD103⁺ DCs (right) in TdLNs (i) or tumours (j) of M3-9-M^Res1/mCherry-bearing mice 24 h post PBS/VSV^∆M51 (n = 6–13 mice/group, compiled from 2 independent experiments, two-tailed, unpaired t test). k Tumour growth (left) and overall survival (right) of M3-9-M^Res1-bearing C57BL/6 or Batf3−/− mice post VSV^∆M51 (left: n = 3–6 mice/group, representative of 2 independent experiments; right: n = 6–12 mice/group, compiled from 2 independent experiments, log-rank Mantel-Cox test). In all experiment, VSV^∆M51 was delivered i.v. at 5 × 10⁸ PFU. Points/bars at mean ± SD. Occasionally n is given as a range as the number of mice in each group varies. Please see the Source Data file for the exact n in each group.