Fig. 2: Development and characterization of CTR 1.0 in S. coelicolor.
From: Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces
a Schematics of CRISPR-based transcriptional repressor 1.0 (CTR 1.0) in this work. The Cascade consists of 5 genes (casA-casB-cas7-cas5-cas6). b CrRNAs targeting the act-orf4 coding region and promoter region of the ACT BGC are shown. c Repression results for targeting the act-orf4 coding sequence using the CTR 1.0. d CrRNAs targeting the red Q gene region of the RED BGC are shown. e Repression results for targeting the redQ coding sequence using the CTR 1.0. f Repression results for targeting both the redQ and act-orf4 genes using the CTR 1.0. Control (The wild-type strain of S. coelicolor).
