Fig. 1: Expression of the cGAS-STING pathway in BAS patients by single-cell RNA sequencing.
From: Macrophage STING signaling promotes fibrosis in benign airway stenosis via an IL6-STAT3 pathway
a Schematic overview of the experimental design. Single-cell RNA sequencing of Normal group (n = 3, cell=31,478) and Granulation group (n = 4, cell=27108). b UMAP visualization of Normal group and Granulation group (58586 cells in total). c UMAP visualization of 14 cell types (58586 cells in total). d Heatmap of expression for three representative marker genes of each cell type. e Pie chart of the total proportion of each cell type identified in Normal group and Granulation group. f GSEA analysis of increasingly expressed genes showing TOP 10 of enriched GO terms in Granulation group compared with Normal group. g GSEA analysis showing significant enrichment of gene sets associated with cytosolic DNA sensor pathway in Granulation group compared with Normal group. h UMAP visualization of STING and cGAS expression in all clusters. i Dot plot showing the expression of cGAS-STING pathway 9 associated genes (cGAS, STING, TBK1,NFKB1, IRF3, CXCL10,CCL4,IL1B,IL6) in all clusters. j UMAP visualization of cGAS-STING pathway score in all clusters. k Box Plot showing the cGAS-STING pathway score in Normal group (n = 31,478 cells) and Granulation group (n = 27,108 cell). l Box Plot showing the cGAS-STING pathway score in all clusters of Granulation group: B cells (n = 1329), basal cells (n = 3790), ciliated cells (n = 25), endothelial cells (n = 580), epithelial cells (n = 574), fibroblasts (n = 2004), mast cells (n = 39), macrophages (n = 4409), neutrophils (n = 9977), plasma cells (n = 819), proliferating cells (n = 619), SMCs (n = 1097) and T cells (n = 1846). n represents the number of cells in cluster. In (k, l), box bounds shows 25th and 75th percentiles, whiskers shows 25th percentiles minus 1.5*IQR(Interquartile Range, the value is 75th percentiles minus 25th percentiles) to 75th percentiles plus 1.5*IQR and box center shows the median.The two-sided Mann Whitney test was used in (k). One-way ANOVA analysis followed by Duncan multiple- range test was used in (l). Source data are provided as a Source Data file.
