Fig. 5: STING expression in macrophages in BAS is associated with inflammation.
From: Macrophage STING signaling promotes fibrosis in benign airway stenosis via an IL6-STAT3 pathway
a Heatmap showing that the BAS group upregulated genes involved in cGAS,STING and inflammation factors compare to control group at 24 h after tracheal injury (n = 3 mice per group). b–f Mice were divided into three groups: control, BAS + PBS and BAS + C176. b Schematic of C176 administration in mice. c Representative images of H&E staining in different groups. Scale bars indicate 200 μm and 50 μm. d Quantitative comparison of lung function indices (Inspiratory time) in different groups (n = 4 mice per group). e mRNA levels of IL6, IL1β and Cxcl10 in trachea from different groups (n = 4 mice per group). f Protein levels of inflammation factors (IL6,CCL12,CCL4,CXCL5 and GM-CSF)in trachea from different groups (n = 6 mice per control group, n = 8 mice per BAS group). g–m sc-RNA seq analysis of macrophage clusters. g UMAP visualization of macrophage clusters in Normal group (1216 cells in total) and Granulation group (4409 cells in total). h UMAP visualization of Seven types of macrophages (5625 cells in total). i Barplot of the percentage of each type in Normal group and Granulation group. j Heatmap of expression for three representative marker genes of each cell type. k Dot plot of the enriched Gene ontology (GO) biological processes of highly expressed genes in each cluster. Dot size corresponds to the proportion of cells within the group expressing each gene, and dot color correspond to GO enrichment p-value. l Box Plot showing the cGAS-STING pathway score in all clusters:Mc1 (n = 2945), Mc2(n = 1176), Mc3 (n = 881), Mc4 (n = 245), Mc5 (n = 172), Mc6 (n = 110), Mc7 (n = 96). n represents the number of cells in cluster. m UMAP visualization of cGAS-STING pathway score and the expression of STING,cGAS,IL1B,IL6 and CXCL10 in all clusters. In (l), box bounds shows 25th and 75th percentiles, whiskers shows 25th percentiles minus 1.5*IQR to 75th percentiles plus 1.5*IQR and box center shows the median. Data are presented as the mean ± SEM. One-way ANOVA analysis followed by Tukey post hoc multi-comparison test was used in (d, e). Kruskal waliis test used in (f). Hypergeometric test was used in (k). Source data are provided as a Source Data file.
