Fig. 3: CRISPR-CasRx treatment rescues stressor-induced neurotoxicity in C9orf72 patient iPSC-derived spinal neurons (iPSC-SNs). | Nature Communications

Fig. 3: CRISPR-CasRx treatment rescues stressor-induced neurotoxicity in C9orf72 patient iPSC-derived spinal neurons (iPSC-SNs).

From: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo

Fig. 3

a Glutamate-induced toxicity was assessed 20 days after nucleofection with CRISPR-CasRx plasmids. b, c Cell death in control and C9orf72 iPSC-SNs expressing CRISPR-CasRx plasmids and single gRNAs, quantified as the ratio of propidium iodide (PI) positive spots to DAPI positive nuclei and Alamar Blue cell viability assay following 4-h exposure to 10 µM glutamate. n = 6 lines per condition. d Schematic of glutamate-induced excitotoxicity assay in control and C9 lines expressing CRISPR-CasRx dual guide plasmids. e, f Quantification of PI incorporation and Alamar Blue cell viability following 4-h exposure to 10 µM glutamate, demonstrating rescue by the dual guide CRISPR-CasRx. Data points for PI incorporation represent average percent cell death across 10 images per well. Data points for Alamar Blue assay represent average percent viability from 3 replicate wells for each condition. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. Data presented as mean ± S.D. Source data are provided as a Source Data file.

Back to article page