Fig. 3: Analysis of SCCs purified from S. mutans strains.

Quantitative comparison of the linkage groups in WT SCC with (A) ΔsccN SCC, (B) ΔsccP SCC, (C) ΔsccM SCC and (D) ΔsccQ SCC and (E) ΔsccMΔsccQ SCC. F Recovery of non-reducing terminal Glc in SCCs purified from S. mutans strains. For this illustration, data are expressed as number of molecules normalized to a total of 50 rhamnose (Rha) per polymer (the proposed average size of the polyrhamnose backbone). The raw data for A–F are presented in Supplementary Table 2. G Phosphate content of SCCs isolated from S. mutans strains. Phosphate concentration was determined by the malachite green method following digestion with perchloric acid. Rha content was determined by a modified anthrone assay, as described in Methods. Data are expressed as nmol of phosphate in the sample normalized to 50 nmol Rha per molecule of SCC. In A–G, columns and error bars represent the mean and S.D., respectively (n = 3 independent replicates). P values were calculated by two-way ANOVA with Dunnett’s multiple comparison test. Data used for plots shown in A–F are presented in Supplementary Table 2. H Fluorescence imaging analysis of SDS-PAGE of ANDS-labeled SCCs prepared from the indicated S. mutans strains. Representative image from at least three independent experiments is shown. Source data for g and h are provided as a Source data file.