Fig. 5: Selective responses to novel objects in ovoid cells.

A Viral and imaging strategy for assessing activity in ovoid neurons (top) and pyramidal neurons (bottom) with 1-photon calcium imaging, with approximate GRIN lens placement. B Example fields of view with background-removed activity maps (left) as well as maximum projections with extracted segmented cells (right), for both ovoid cells (top row) and pyramidal cells (bottom row). Scale bar: 100 µm. This was independently repeated 12 times. C Example calcium traces for ovoid neurons (top) and pyramidal neurons (bottom) taken during exploration in the novel object-encoding session. Scale bar: 10 s, with y-axis having arbitrary units. D Schematic of novel object recognition paradigm. E Object-interaction-triggered ovoid neuron activity for novel and familiar object interactions, for a representative animal undergoing the behavioral paradigm illustrated in (D). Vertical black line at 0 denotes start of interaction with an object, with individual lines illustrating averaged activity of all segmented individual cells within the field of view for the session. F As in (E), but for a representative animal assessing pyramidal cell activity. G Depiction of classification of responder cells, wherein cells must reach a minimum ΔZ of 0.25 for a minimum of 10 s following object interactions. H The percentage of cells that classify as a responder cell for novel (red) and familiar (blue) objects, for ovoid cells (left) and pyramidal cells (right). Data points represent individual animals (n = 6 animals for each of ovoid and pyramidal conditions, ovoid: p = 3.1e-3, pyramidal: p = 0.48, two-sided linear model, no adjustment for multiple comparisons) and data are presented as mean values ± SEM.