Fig. 1: ReSTED using fluorescence-recoverable nanographene.
From: Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene
a Normalized absorption and emission spectra of DBOV-Mes (µM in toluene) and absorption spectrum of DBOV-Mes•+ after the addition of 10 equivalents of SbCl5 to a solution of DBOV-Mes in dry dichloromethane. Excitation (Exc.) beam: 561 nm; STED beam: 775 nm; detection range: 600–750 nm. Confocal images of cracks from a coverslip with DBOV-Mes before (b) and after (c) deactivation by excitation beam and after reactivation by the reactivation beam (d). White dashed square region – region of interest (ROI)1: deactivated area. Orange dashed square region—ROI2: reactivation beam scanned area. Scale bars: 2 µm. The color bars represents a linear scale. e Diagrams illustrating molecular states (ground state DBOV-Mes, fluorescent excited state DBOV-Mes*, and non-fluorescent deactivated state DBOV-Mes•+) of DBOV-Mes for ReSTED imaging. All the parameters are summarized in Supplementary Note 1. Source data are provided as a Source Data file.
