Fig. 3: Reactivation properties of DBOV-Mes.
From: Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene
a, b Confocal images of before (upper panel) and after (lower panel) confocal/STED imaging. The ROI in red dashed boxes was imaged by confocal and STED imaging for 5 frames. Scale bars: 5 µm. The color bars represent a linear scale. c The leftover fluorescence ratio after confocal and STED imaging over the number of imaging frames/time. d The leftover fluorescence intensity dependent on the reactivation beam power density while keeping the excitation intensity constant. e The reactivation properties of DBOV-Mes measured under different imaging conditions (air, water, or PBS buffer). Error bars in b-d represent standard deviations calculated from 3 or 4 measurements. All the experimental settings/parameters are summarized in Supplementary Note 1. Source data are provided as a Source Data file.
